金钗石斛叶片2个发育阶段的转录组比较分析OA

[Objective]The expression characteristics of differentially expressed genes in Dendrobium nobile leaves at two development stages were analyzed to provide a reference for growth and development of D.nobile.[Method]The transcriptome data and gene expression characteristics of D.nobile leaves at two development stages were analyzed by using Illumina high-throughput sequencing technology to identify the gene expression pattern of D.nobile leaves at different development stage.[Result]A total of 126 981 single-genes with an average length of 1 035 bp were obtained by transcriptome sequencing.The differentially expression genes with 313 up-regulated genes and 757 down-regulated genes were identified in D.nobile leaves at two different developmental stages.The functional clustering of differentially expressed genes showed that the metabolic pathways and biosynthesis of secondary metabolites may be closely related to D.nobile leaf development.In addition,9 917 single-genes of encoding transcription factors were identified and subject to 57 gene families.Among them,there were 335 MYB transcripts.Two up-regulated and five down-regulated MYB genes were identified in D.nobile leaves by analyzing their expression profiles at two developmental stages.[Conclusion]The transcriptome comparative analysis of D.nobile leaves at two developmental stages shows that the number of down-regulated genes is significantly higher than that of up-regulated genes,and members of MYB,MADS,NAC and WRKY transcription factor families are identified.