基于多层次加权评判法和聚类分析法的草珊瑚种质资源评价OA

[Objective]The potential excellent Sarcandra glabra germplasm resources were screened by comprehensively evaluating S.glabra germplasm resources to provide a reference for breeding,development and utilization of improved S.glabra germplasm varieties.[Method]The agronomic traits(plant height,ground diameter,stem number,leaf length and leaf width),chlorophyll relative content index(SPAD value)and medicinal components(isofraxidin and rosmarinic acid)content of 30 S.glabra germplasm resources collected from Guangxi and surrounding areas were investigated and 30 S.glabra germplasm resources were comprehensively evaluated by multi-level analysis,weighted calculation algorithms and hierarchical cluster analysis(HCA).[Result]There were significant differences in phenotype and metabolism among 30 S.glabra germplasm resources.The variable coefficients of plant height,stem number and leaf area were 33.6%,43.8%and 30.2%respectively.The plant height of No.25 was 117.5 cm and 69.31%higher than the average population value(69.4 cm).The leaf area of No.10 was 68.7 cm2 and 1.53 times the average population value(45.0 cm2).There were obvious differences in isofraxidin content(0.008%-0.041%)and rosmarinic acid content(0.020%-1.375%).Total content(1.399%)of medicinal compositions in No.20 was 2.3 times the average population value(0.600%).The comprehensive score of No.22,No.24 and No.33 with good yield traits(plant height≥93.6 cm and stem number≥8.6)and better quality trait(total medicinal components content≥1.573%)was 0.756,0.732 and 0.748 respectively.30 S.glabra germplasm resources were clustered into four groups.9 S.glabra germplasm resources in Group IV with high-yield characteristics and high-quality potential could be used as the excellent materials in single-plant pedigree breeding of S.glabra.[Conclusion]The comprehensive evaluation method including multi-level weighted evaluation and clustering analysis can be used in comprehensive evaluation of S.glabra germplasm resources.Group IV with high-yield and high-quality traits should be used as the excellent materials in single-plant breeding of S.glabra.