水稻ABF转录因子家族的全基因组鉴定及表达模式分析OA

[Objective]The physicochemical properties,structure characteristics and expression pattern of OsABF gene family members in rice were explored and verified to lay a foundation for biological functions of rice OsABF gene family.[Method]The members of rice OsABF family were identified by bioinformatics,and their phylogenetic relationships,gene structure,conserved motifs and collinearity,promoter elements and expression patterns in different tissues were analyzed.The expression patterns of OsABF genes under different abiotic stresses were analyzed by qRT-PCR technology.[Result]11 OsABF genes were identified from rice genome and named as OsABF1-OsABF11.The protein sequence length,size and isoelectric point of OsABF1-OsABF11 were 244-388 amino acids with an average length of 332 amino acids,25.48-41.48 kDa with an average of 35.40 kDa and 5.30-9.56,respectively.11 OsABF protein were divided into two subfamilies of A(OsABF4,OsABF7,OsABF8,OsABF10 and OsABF11)and B(OsABF1,OsABF2,OsABF3,OsABF5,OsABF6 and OsABF9).OsABF was relatively conserved in two subfamilies.The segmental duplication was the main factor driving evolution of rice OsABF gene family and the purifying selection played an important role in the evolution of rice OsABF gene family.The response of 8 plant hormones,regulation of 6 plant growing development and 5 cis-acting elements under abiotic and biological stresses were respectively identified from upstream promoters of OsABF genes,and the sequence of all OsABF promoters had ABRE cis-acting elements.The expression profile analysis showed that expression of most OsABF genes in subfamily A was in different tissues and induced by abiotic stresses such as drought,low temperature,ABA,and JA.[Conclusion]11 OsABF protein are divided into A and B subfamily with a relative conserved function.OsABF4 and OsABF10 in A subfamily play an important role in rice growing development and the response to abiotic stress.