NOP14过表达抑制NRIP1表达及卵巢癌SKOV3细胞的增殖与迁移OA

Objective To investigate the effect of nucleolar protein 14(NOP14)overexpressi on proliferation and migration of ovarian cancer cells SKOV3 and the underlying mecha-nism.Methods The expression level of NOP14 in ovarian cancer cell lines(SKOV3,A2780,HO-8910,OVCAR)was detected by RT-qPCR and Western blotting.SKOV3 cells were transfected with the pcDNA-NOP14 plasmid.Cell proliferation was detected by colony formation assay and 5-E-thynyl-2′-deoxyuridine(EdU)staining.Tumor stem cell characteristics were analyzed via serum-free sphere formation assay.The proportion of CD133-positive(CD133+)cells was measured by flow cytometry.The epithelial-mesenchymal transition(EMT)process was evaluated by observing cell morphological change and detecting the EMT marker proteins(E-cadherin,N-cadherin,Vim-entin).The expression level of nuclear receptor interacting protein 1(NRIP1),and Wnt/β-catenin pathway proteins were detected by RT-qPCR and Western blotting.Results The expression level of NOP14 was significantly lower in the ovarian cancer cell lines than that in the normal ovarian epithe-lial cells.Overexpression of NOP14 significantly inhibited the proliferation,stem cell characteris-tics,and EMT of SKOV3 cells(mesenchymal markers N-cadherin and Vimentin were downregulat-ed,while the epithelial marker E-cadherin was upregulated).Moreover,Overexpression of NOP14 suppressed the expression of NRIP1,Wnt and β-catenin.Conclusion NOP14 can inhibit the pro-liferation,stem cell characteristics,and EMT process of ovarian cancer SKOV3 cells,and nega-tively regulate the expression of NRIP1,Wnt,β-catenin.