金丝桃苷通过NOX4抑制膀胱尿路上皮癌细胞的增殖OA

Objective To analyze the inhibitory effect of hyperoside on inhibiting the prolifera-tion of bladder urothelial carcinoma(BLCA)cells and the mechanism.Methods Cell prolifera-tion was detected by CCK-8 and colony formation assays.The expression level of nicotinamide ade-nine dinucleotide phosphate oxidase 4(NOX4)in cells under different concentration of hyperoside and intervention time,and the expression levels of mTOR,AKT,S6 cells and NOX4 proteins were detected by Western blotting.Results The proliferation ability of cells,number of cell colonies,volume and mass of transplanted tumor tissues,and expression level of Ki67 protein were signifi-cantly decreased in the hyperoside group(P<0.05).The expression level of NOX4 was significant-ly decreased with the increase of the treatment time and concentration of hyperoside(P<0.05).Compared with those in the control group,the expression levels of p-mTOR,p-S6,p-Akt and NOX4,and number of colonies were significantly decreased in the hyperoside group,while the a-bove indexes were significantly increased in the NOX4 overexpression group and the hyperoside+NOX4overexpression group(P<0.05).Conclusion Hyperoside can inhibit BLCA proliferation by supressing the expression of NOX4.