陆地棉和海岛棉种间TCP转录因子比较分析OA

[Objective]To compare the characteristics of TCP gene family members between G.hirsutum and G.barbadense.[Method]Bioinfor-matics methods were used to identify the TCP gene family of G.hirsutum and G.barbadense in the whole genome,and to compare and analyze their physicochemical properties,chromosome localization,systematic evolution and tissue expression.[Result]69 and 70 TCP genes were i-dentified in G.hirsutum and G.barbadense,respectively,with 31 and 34 pairs of homologous genes distributed on 22 and 21 chromosomes,re-spectively,all of which were hydrophilic proteins.59 pairs of homologous genes were identified between G.hirsutum and G.barbadense.The systematic evolutionary relationship showed that it was divided into three subfamilies:CIN,CYC/TB1 and PCF.TCP gene family members of the same subfamily in G.hirsutum and G.barbadense had similar gene structures.Gene tissue-specific expression analysis revealed that the gene Ghir_D13G006190 was expressed in all tissues of G.hirsutum,while the homologous gene Gbar_D13G006010 was not expressed in any tissue of G.barbadense,and belonged to the PCF class in the evolutionary tree.Ghir_A09G000900 was only highly expressed in petals of G.hirsu-tum,while the homologous gene Gbar_A09G000980 had the highest expression levels in petals,anthers and styles of G.barbadense,and be-longs to the CIN class in the evolutionary tree.Ghir_A12G023760 was most highly expressed in leaves of G.hirsutum,while the homologous gene Gbar_A12G023880 was most highly expressed in stems of G.barbadense,and both belong to the PCF class in the evolutionary tree.Cis-acting element analysis showed that the promoters of the TCP gene family of G.hirsutum and G.barbadense contain many regulatory elements related to plant light response,physiological response,hormone response,and stress response.[Conclusion]This study provides an important theoretical basis for further exploring the functional differences of TCP transcription factors between G.hirsutum and G.barbadense.