粳稻花时性状QTL定位与聚合效应分析OA
QTL Mapping and Pyramiding Effect Analysis of Diurnal Floret Opening Time Traits in japonica Rice
[目的]水稻日开花时间是影响粳稻不育系异交率及制繁种产量的重要性状.本研究旨在定位粳稻花时调控相关的 QTL 并对其聚合效应加以分析,明确早花时 QTL 的主效位点.[方法]利用早花时粳稻保持系粳65B(J65B)和晚花时粳稻保持系粳 139B(J139B)构建包含221 个家系的重组自交系群体.连续 3 年调查群体花时性状,采用遗传图谱和BSA-seq两种方法进行花时QTL定位分析.[结果]利用完备区间作图法共检测到 30 个花时性状QTL,分布于 2～12号染色体上,其中qFT5.2、qFT5.3、qFT5.5、qFT10.2、qFT10.3 在 8 个以上环境中被同时检测到,加性效应范围 13.04～50.93,是稳定表达的主效位点;选取花时极端早和极端晚家系构建混池,利用BSA-seq方法在qFT5.3、qFT5.5、qFT6.2、qFT10.2 位点检测到花时关联区域;选取两种方法共同定位到的稳定表达位点进行QTL聚合分析,J65B类型的QTL均会提早花时,qFT5.3 和qFT6.2 是早花时改良的基础位点,qFT5.5、qFT10.2 是进一步提早花时的重要位点.[结论]采用两种方法定位到 4 个稳定表达主效花时QTL qFT5.3、qFT5.5、qFT6.2 和qFT10.2,为早花时基因进一步精细定位、基因克隆和粳稻花时性状改良奠定了基础.
[Objective]Diurnal flowering time(FT)of rice is an important trait that affects the outcrossing rate and seed production yield of japonica rice sterile lines.This study aims to locate QTLs related to diurnal-floret-opening-time(DFOT)in japonica rice and analyze the pyramiding effects to clarify the main effect loci for DFOT traits.[Method]Recombinant inbred line populations consisting of 221 lines were constructed using japonica maintainer line J65B with early floret-opening-time and J139B with late floret-opening-time.The floret-opening-time was investigated in three years.QTL mapping analysis was conducted using genetic mapping and BSA-seq methods.[Result]A total of 30 DFOT QTLs were detected using complete interval mapping(ICIM)method on chromosomes 2-12.qFT5.2,qFT5.3,qFT5.5,qFT10.2,and qFT10.3 were major and stable QTLs which were detected simultaneously in more than eight environments,with an additive effect range of 13.04-50.93.Based on floret-opening time,extreme individuals were pooled for BSA-seq analysis.Association intervals related to floret-opening-time were detected in the regions of qFT5.3,qFT5.5,qFT6.2,and qFT10.2.According to the genotyping results,different progenies were divided into J139B type and J65B type in each QTL interval.QTL pyramiding effect analysis showed that QTLs derived from J65B would advance diurnal-floret-opening-time.The loci of qFT5.3 and qFT6.2 were essential for achieving early diurnal-floret-opening-time.The loci of qFT5.5 and qFT10.2 could further advance the diurnal-floret-opening-time.[Conclusion]Genetic mapping and BSA-seq analysis successfully identified four major stable QTLs related to rice diurnal-floret-opening-time.The localization of qFT5.3,qFT5.5,qFT6.2,and qFT10.2 establishes a foundation for further fine mapping and gene cloning and genetic improvement of diurnal-floret-opening-time in japonica rice.
张城;孙廉平;邵国军;张雪;田书军;孙驰;郭艳颖;周燃;韩勇;郑文静
辽宁省水稻研究所,沈阳 110101中国水稻研究所/国家水稻改良中心/水稻生物育种全国重点实验室,杭州 311401辽宁省水稻研究所,沈阳 110101辽宁省水稻研究所,沈阳 110101辽宁省水稻研究所,沈阳 110101辽宁省水稻研究所,沈阳 110101辽宁省水稻研究所,沈阳 110101中国水稻研究所/国家水稻改良中心/水稻生物育种全国重点实验室,杭州 311401辽宁省水稻研究所,沈阳 110101辽宁省水稻研究所,沈阳 110101
粳稻花时重组自交系BSA-seqQTL定位
japonica ricediurnal-floret-opening-timerecombinant inbred lineBSA-seqQTL mapping
《中国水稻科学》 2026 (1)
106-117,12
辽宁省自然科学基金面上项目"杂交粳稻保持系早花时QTL定位"(2022-MS-059)辽宁省农业科学院学科建设计划项目(2024XKJS5222).
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