大花地不容生物碱成分研究OA

Various chromatographic techniques,including normal and reversed-phase silica gel column chromatography as well as semi-preparative HPLC,were employed to separate and purify the total alkaloids from Stephania macrantha,resulting in the isolation of six alkaloids.Their structures were elucidated using a combination of 1D and 2D NMR techniques,high-resolution mass spectrometry,circular dichroism spectroscopy,quantum chemical calculations,and acid hydrolysis methods.The six alkaloids were identified as isothebaine-1-O-β-D-glucopyranoside(1),isocorydine(2),stepharine(3),coclaurine(4),reticuline(5),and coclaurine(6),with compound 1 being a novel alkaloid glycoside.The anti-proliferative activity of the isolated alkaloids against four tumor cell lines(Hep G2,A549,FaDu,HGC-27)was assessed using the CCK-8 method.The results demonstrated that isocorydine(2)exhibited significant inhibitory effects on the growth of HGC-27 tumor cells,with IC50 values of 17.02 µM.