不同产地牛舌草对皮质酮诱导HT-22细胞损伤的神经保护作用OA

Based on the traditional clinical efficacy and modern pharmacological research of Anchusa italica Retz.,the pharmacological effects and material basis of Anchusa italica Retz.were studied systematically by constructing an in vitro depression model.The chemical constituents of total flavonoid of Anchusa italica(TFAI)from different habitats(Xinjiang,Pakistan,Hainan)were detected by high performance liquid chromatography(HPLC).The hippocampal cells(HT-22)were used as the research object,and the nerve cell injury model was induced by corticosterone.The effects of five kinds of Anchusa italica Retz.on the viability of HT-22 cells were detected by MTT method,and the EC50 was calculated.The results showed that TFAI from different habitats had no obvious cytotoxic effect on HT-22 cells at a concentration of 500 µg/mL,and had a significant proliferation effect on HT-22 cells,and had a significant cytoprotective effect on hippocampal cell damage induced by excessive corticosterone.Compared with the flavonoid content and EC50 value of 5 batches of Anchusa italica Retz.,the flavonoid content in Anchusa italica Retz.from Hainan was the highest,and its EC50 value on HT-22 cell proliferation was the lowest,with a high effect value.The results show that the five batches of TFAI extracts have a protective effect on corticosterone-induced HT-22 hippocampal cell injury,which can increase the viability of HT-22 cells to varying degrees and have a significant neuroprotective effect.