长叶格脉树乙醇提取部改善L6细胞葡萄糖摄取的作用机理OA

Garcinia species have historically been known for their diversity of chemical constituents and richness of pharmacological activities.The research progress on the chemical components of Ochrocarpus longifolius is extremely lacking in the market.Ochrocarpus longifolius has entered the medicinal market as a substitute for Mesua ferrea L.,and its potential pharmacological activity cannot be underestimated.To study the anti-diabetic activity of ethanol extract of Ochrocarpus longifolius(OLEE),L6 cells were used to test the ability of OLEE to promote glucose uptake,and then L6 cells stably expressing myc epitope tag were used to observe the ability of OLEE to promote GLUT4 fusion with the plasma membrane by laser confocal microscopy.Finally,Western blot was used to detect the expression of GLUT4 and related signaling pathways in L6 cells under OLEE treatment.Test results indicate that OLEE stimulation enhanced glucose uptake and promoted the fusion of GLUT4 to the plasma membrane in L6 cells.At the protein level,OLEE enhanced the expression of GLUT4 and the phosphorylation levels of AMPK and PKC.These results provide strong evidence and basis for OLEE as a new anti-diabetes drug in the future.