模拟苏打碱处理对不同品种大豆抗逆性比较研究OA
土壤盐渍化日益严重,已经在多方面严重影响粮食生产和安全,改善土壤盐渍化刻不容缓.取各生长 2 周后的大豆幼苗,模拟苏打土环境,4 种大豆分别在 0、100、200 mmol/L 的等摩尔Na2CO3 和NaHCO3 碱溶液进行野大豆、9316 号、97 号与白农 6号幼苗胁迫处理 72h后,取叶与根,检测材料中的脯氨酸(Proline,PRO)含量、丙二醛(Malondialdehyde,MDA)含量、过氧化氢酶(Catalase,CAT)酶活和总抗氧化能力 4 项生理指标.经统计分析,结果表明,不同大豆品种、不同的部位其耐盐效果存在明显差异.叶片中MDA含量和PRO含量随着胁迫溶液浓度增大而增大;CAT酶活除 9316 号,其余 3 种大豆的MDA随着胁迫溶液浓度增大而减小;总抗氧化酶能力中野大豆和 9316号随着胁迫溶液浓度增大先减小后增大,97 号和白农 6 号大豆则随着胁迫溶液浓度增大而增大.在根系中CAT和PRO含量随着胁迫溶液浓度增大而增大;丙二醛和总抗氧化能力则随着胁迫溶液的浓度增大而减小.通过以上分析,4 种大豆中 9316 号具有较高的 MDA和PRO含量、较强的CAT活力、总抗氧化能力较强,其耐盐性相比其他 3 种大豆品种较强,抗逆效果最佳.
Soil salinization is becoming increasingly serious.It has seriously affected food production and security in many aspects.It is urgent to improve soil salinization.Soybean seedlings after growing for 2 weeks were taken to simulate a soda soil environment.After the seedlings of wild soybean,No.9316,No.97 and Bainong 6 were treated with equal molar Na2CO3 and NaHCO3 alkali solutions for 72 hours,the leaves and roots of the four soybeans were taken to detect proline in the materials Four physiological indicators:Proline(PRO)content,malondialdehyde(MDA)content,catalase(CAT)activity,and total antioxidant capacity.After statistical analysis,the results showed that there were significant differences in salt tolerance among different soybean varieties and different parts.The MDA content and PRO content in leaves increased with the increase of stress solution concentration;except for CAT enzyme activity in No.9316,the MDA of the other three soybeans decreased with the increase of stress solution concentration;the total antioxidant enzyme capacity of Zhongye Soybean and No.9316 decreased first and then increased with the increase of stress solution concentration,while No.97 and Bainong 6 increased with the increase of stress solution concentration.CAT and PRO contents in roots increased with the increase of stress solution concentration,while MDA and total antioxidant capacity decreased with the increase of stress solution concentration.Through the above analysis,among the four soybean varieties,9316 has higher MDA and PRO content,stronger CAT activity,and stronger total antioxidant capacity.Compared with the other three soybean varieties,its salt tolerance is stronger,and its stress resistance effect is the best.
丛建民;葛梦;尹欣幸;赵国富;李海林;黄威剑;苏江顺;赵永
台州科技职业学院 农业与生物工程学院,浙江 台州 318020||台州科技职业学院 作物研究所,浙江 台州 318020白城师范学院 生命科学学院,吉林 白城 137000台州科技职业学院 农业与生物工程学院,浙江 台州 318020||台州科技职业学院 作物研究所,浙江 台州 318020台州科技职业学院 农业与生物工程学院,浙江 台州 318020||台州科技职业学院 作物研究所,浙江 台州 318020台州科技职业学院 农业与生物工程学院,浙江 台州 318020||台州科技职业学院 作物研究所,浙江 台州 318020台州科技职业学院 农业与生物工程学院,浙江 台州 318020||台州科技职业学院 作物研究所,浙江 台州 318020白城市农业科学院,吉林 白城 137000白城师范学院 生命科学学院,吉林 白城 137000
农业科技
大豆碱胁迫生理响应抗逆性品种筛选
soybeanalkali stressphysiological responsestress resistancevariety selection
《智慧农业导刊》 2026 (2)
25-30,6
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