动物病原微生物高通量靶向测序技术(扩增法)规范专家共识OA

Targeted next-generation sequencing(tNGS)-based diagnostic technologies enable the rapid acquisition of pathogen sequence information from samples,allowing for the detection and identification,source tracing and genotyping,and comprehensive characterization of animal pathogenic microorganisms,including certain zoonotic pathogens.Therefore,the development of this technology serves not only as a valuable complement to existing nucleic acid-based diagnostic methods for animal diseases,but also as an important technical safeguard for veterinary public health security and the sustained,stable development of the livestock industry.Given that a unified and standardized consensus has not yet been established within the veterinary diagnostic field,this consensus document systematically addresses the application scenarios of tNGS in animal pathogen detection,basic laboratory requirements,guiding principles for primer panel design,experimental workflows,data analysis,result description and interpretation,and sequencing reports,and proposes standardized requirements and practical recommendations.