miRNA-133调节hTim8a对高糖诱导的肾小管上皮细胞损伤的影响OA

Objective:To investigate the effect of miRNA-133 on high glucose induced renal tubular epithelial cell injury by regulating human translocase of inner mitochondrial membrane 8a(hTim8a).Methods:Rat renal tubular epithelial cells HK-2 were cultured in vitro and divided into CK group,high glucose group,high glucose+inhibitor-NC group,and high glucose+miRNA-133 in-hibitor group.qRT-PCR was used to detect expressions of miRNA-133 and TIMMA genes in cells.MTT method was used to detect cell proliferation.Flow cytometry was used to detect cell apoptosis rate.Biochemical methods were used to detect ROS and ATP levels.ELI-SA kits were used to detect LDH,TNF-α,IL-6 and IL-10 levels.Commercialized reagent kits were used to detect MDA,SOD and CAT levels.Western blot was used to detect expressions of cleaved caspase-3,hTim8a,mt-COX,PGC-1 and PRC proteins in cells.Results:Compared with CK group,miRNA-133 expression,apoptosis rate,levels of ROS,LDH,TNF-α,IL-6,MDA content,and expression of cleaved caspase-3 protein in HK-2 cells in high glucose group were greatly increased,while the expression of TIMMA mRNA,A490(24 h,48 h)values,levels of ATP and IL-10,activities of SOD and CAT,protein levels of hTim8a,mt-COX,PGC-1,PRC were greatly reduced(P<0.05).Compared with high glucose+inhibitor-NC group,miRNA-133 expression,apoptosis rate,levels of ROS,LDH,TNF-α,IL-6,MDA content,and protein expression of cleaved caspase-3 in HK-2 cells in high glucose+miRNA-133 inhibitor group were greatly reduced,TIMMA mRNA expression,A490(24 h,48 h)values,levels of ATP and IL-10 levels,activities of SOD and CAT,protein levels of hTim8a,mt-COX,PGC-1,PRC were greatly increased(P<0.05).Conclusion:Silencing expression of miRNA-133 can up-regulate the expression of hTim8a,alleviate high-glucose induced inflammation,oxidative stress,and apoptosis in HK-2 cells,thereby improving cell activity and mitochondrial function,and reducing cell injury.