耐受性树突状细胞对胶原诱导性关节炎大鼠肠道菌群结构的影响OA

Objective:To investigate effect of tolerogenic dendritic cells(tolDC)on gut microbiota structure of rats with colla-gen-induced arthritis(CIA).Methods:Experimental animals were randomly divided into Control group,CIA group and tolDC group.CIA group and tolDC group were immunized with bovine type Ⅱ collagen solution to establish CIA model.TolDC group was treated with tolDC via tail vein on 21th day after initial immunization.Arthritis score and thickness of paws in each group of rats were mea-sured weekly.On the 35th day,rats were sacrificed and specimens were taken for testing.HE staining was performed on ankle joint to evaluate pathological changes in each group of rats.16S rDNA sequencing was used to detect changes in gut microbiota structure of rats in each group,including alpha diversity,beta diversity,species composition and species difference analysis.Results:Compared with Control group,arthritis score and thickness of paws in CIA group were increased,exhibited abnormal proliferation and extensive infiltration of inflammatory cells,as well as formation of pannus and destruction of bone and cartilage,intestinal flora index of Chao1 and Shannon were decreased,but difference was not significant,abundance of Firmicutes was decreased,abundance of Clostridioides and Muribaculum were increased.After tolDC treatment,arthritis score and thickness of paws of CIA rats were decreased,infiltration of inflammatory cells,abnormal hyperplasia of synovial tissue,formation of pannus and injury of bone and cartilage were alleviated,intestinal flora index of Chao1 was decreased(P<0.01),abundance of Firmicutes,Lactobacillus,Lachnoclostridium,GCA-900066575,Dubosiella,Peptococcus,Acetatifactor and Butyricicoccus were increased.Conclusion:TolDC alleviates joint injury in CIA rats and influences gut microbiota structure.