不同杂交组合黄羽肉鸡皮肤毛囊密度差异及其与Wnt3a表达水平的相关性分析OA

The present study was conducted with the objective of investigating the differences in skin feather follicle density and its correlation with Wnt3a mRNA expression in different hybrid combinations of yellow-feathered chickens.The experiment was conducted on the basis of four specialized strains,which were bred from local native chicken breeds and fast-sized yellow chicken resources.A total of six binary hybrid combinations(WT,RT,KW,TW,KR and TR)were designed by incomplete double-row crosses.The skin follicle density and Wnt3a mRNA expression levels of diverse skin regions(i.e.back,breast and thigh)of these hybrid combinations were systematically assessed,and a comprehensive correlation analysis was conducted.The results demonstrated that:1)The body weights of the different hybrids were not significantly different(P＞0.05),and the body weights of the hybrids sired by fast-sized yellow chicken(W and R strains)were higher than the others.2)The feather follicle densities of the various hybrids exhibited significant variation(P＜0.05),with the follicle density of the hybrids sired by the local breeds(K and T strains)being higher than that of the other hybrids.3)A statistically significant discrepancy(P＜0.05)was identified in the feather follicle density and Wnt3a mRNA expression levels among the distinct anatomical regions of the hybrid subjects.A gradient distribution pattern was observed,with higher levels in the back region followed by the breast and then the thigh.A positive correlation was identified between follicle density and Wnt3a mRNA expression,with varying degrees across different hybrids.4)A negative correlation was identified between feather follicle density and body weight with varying degrees across different hybrids.In summary,the present study compared the differences in skin feather follicle density among different hybrid combinations of yellow-feathered chickens,confirming the regulatory role of the Wnt3a gene in feather follicle development and revealing the potential existence of a genetic antagonism between growth rate and feather follicle development,which provided a theoretical basis for the molecular breeding strategy to balance growth rate and carcass appearance traits in the selection and breeding of high-quality yellow-feathered chickens.