新藤黄酸对喉癌细胞增殖、迁移的抑制作用及其机制研究OA

Objective To investigate the effect of gambogenic acid on the proliferation and migration of laryngeal cancer Tu686 cells and its mechanism.Methods The effects of gambogenic acid on the growth and migration of Tu686 cells were analyzed by MTT assay,plate cloning assay,wound healing assay,Transwell assay and Hoechst 33258 staining.The molecular targets of gambogenic acid on laryngeal cancer cells was predicted by Network pharmacology and molecular docking,and the expression levels of related proteins were detected by Western blot.Results Gambogenic acid significantly inhibited the proliferation,migration and invasion of Tu686 cells.Network pharmacology screening identified 19 common targets of gambogenic acid-laryngeal cancer.KEGG enrichment analysis suggested that gambogenic acid might inhibit tumor progression by regulating cancer-related pathways.Molecular docking results showed that gambogenic acid had strong binding activity to the core targets.Hoechst 33258 staining and Western blot results showed that gambogenic acid could induce apoptosis of Tu686,down-regulate the expression of STAT3 and BCL2 proteins and up-regulate the expression of BAX protein.Conclusion Gambogenic acid can significantly inhibit the proliferation and migration of laryngeal cancer Tu686 cells,which may be related to multiple targets.