高致病性猪繁殖与呼吸综合征活疫苗和猪圆环病毒2型灭活疫苗联合免疫效果的评价OA

To establish a convenient and effective co-immunization method for highly pathogenic porcine reproductive and respiratory syn-drome(HP-PRRS)and porcine circovirus type 2(PCV2),this study evaluated the feasibility of combined vaccination through in vitro cell infection and pig immunization experiments.The test group received a single intramuscular injection of HP-PRRS live vaccine diluted with PCV2 inactivated vaccine,while the control group received separate injections at two sites;and their antibody responses were monitored u-sing commercial ELISA kits.The results showed that the HP-PRRS virus titers remained stable within one hour after mixing.For HP-PRRS antibodies,the test group exhibited faster antibody elevation at 30 days post-immunization,with a significantly higher mean S/P value(2.01)at 60 days,compared with the control group(1.55).For PCV2,the antibody levels increased in both groups,reaching 2.16(the test group)and 2.29(the control group)at 90 days,respectively,with no significant difference(P>0.05).The test group demonstrated more uniform HP-PRRS antibody responses but slightly dispersed PCV2 antibodies.The statistical analysis confirmed no interference in anti-body production between the two vaccines,with comparable efficacy between the groups.This study validated the feasibility of co-administe-ring HP-PRRS live vaccine and PCV2 inactivated vaccine,providing a practical solution for field immunization.