牛源林氏棒状杆菌分离株LSKT01鉴定及全基因组测序OA

The aim of this study was to isolate and identify the suspected Corynebacterium hindlerae,to analyze its whole genome character-istics.The tissue samples of suspected patients in a dairy farm in Kuitun,Xinjiang were collected aseptically.Then,the suspected colonies were identified by culture characteristics,biochemical characteristics,Gram staining and transmission electron microscopy.Next,the purified bacterial liquid was identified by 16S rRNA and echA8 gene PCR.Finally,the drug sensitivity test of the isolated bacteria was carried out using the micro-broth dilution method,and Illumina TruSeq sequencing was performed for genome assembly and annotation-related bioinfor-matics analysis.The results showed that a Corynebacterium strain was successfully isolated;it was identified as Corynebacterium lindtii by 16S rRNA and echA8 genes,and the homology of the strain with GenBank was 98.64％-99.86％.The results of the phylogenetic tree showed that the isolated bacteria and Corynebacterium lindleyi were in the same evolutionary branch and had the closest genetic relationship.The results of the drug sensitivity showed that the isolates were highly sensitive to vancomycin,gentamicin,linezolid,tetracycline and doxycycline,were moderately sensitive to erythromycin,penicillin and rifampicin,and were resistant to clindamycin,moxifloxacin,ciprofloxacin,cefotaxime and ceftriaxone.The sequencing results showed that the whole genome size of the isolate LSKT01 was 2 608 509 bp,and its average G+C content was 58.81％.There were 2 482 predicted coding genes,including 52 tRNAs,1 5S rRNA,1 16S rRNA,1 23S rRNA,1 CRISPR- Cas and 15 prophages.The COG,GO,KEGG,NR and Swiss-Prot databases were used to annotate the gene function of the coding genes which annotated 2 033,846,1 353,2 419 and 1 513 genes,respectively.In summary,a strain of Corynebacterium hindlerae was successfully isolated and identified in this study,and the genetic information of the LSKT01 strain was revealed.