猪血浆中替米考星高效液相色谱检测方法的建立OA
Development of a high-performance liquid chromatography method for the quantification of tilmicosin in porcine plasma
旨在建立一种用于猪血浆中替米考星含量的高效液相色谱(high-performance liquid chromatography,HPLC)检测方法.制备含替米考星标准品的健康仔猪血浆样品,用甲醇提取和氯仿萃取,使用Agilent ZOR BAX Eclipse XDB-C18 色谱柱(4.6 mm×250 mm,5 μm)洗脱,外标法定量分析.结果:方法学验证显示,替米考星在0.15～50 μg/mL的浓度范围内,呈线性(y=9 956.3x-798.18,R2=0.999),日内与日间精密度分别为0.30％～2.46％、1.25％～4.78％,回收率为 85.37％～118.45％.结论:该方法特异性强、灵敏度高、重复性好,适用于猪血浆中替米考星的含量测定.
To develop a high-performance liquid chromatography(HPLC)method for quantifying tilmicosin in porcine plasma,tilmicosin standards were spiked into plasma samples obtained from healthy piglets,followed by extraction with methanol and chloroform.Chromato-graphic separation was achieved on an Agilent ZOR BAX Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm)under gradient elution con-ditions,with quantification performed via an external standard calibration curve.The results were that method validation exhibited excellent linearity(R2=0.999)across the concentration range of 0.15-50 μg/mL,as described by the regression equation y=9 956.3x-798.18.The intra-day and inter-day precision values were 0.30％-2.46％and 1.25％-4.78％,respectively,with recovery rates ranging from 85.37％to 118.45％.In conclusion,the above results indicated that the present method showed superior specificity,sensitivity and repro-ducibility,confirming its appropriate application to the accurate determination of tilmicosin in porcine plasma.
赵莎莎;郭大伟;程星星;龚佳豪;汪宇涵;赵源;司星星;张梦醒;魏经阁;高修歌
江苏农牧科技职业学院,江苏 泰州 225300南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014南京农业大学动物医学院,江苏 南京 210014
农业科技
替米考星高效液相色谱血浆猪外标法
tilmicosinHPLCplasmapigsexternal standard method
《畜牧与兽医》 2026 (2)
56-60,5
江苏农牧科技职业学院科技项目(NSF2024ZR03)
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