甘草多糖对脂多糖诱导的小鼠急性肺炎的保护作用OA

The aim of this study was to explore the protective effect of Glycyrrhiza polysaccharide(GCP)on lipopolysaccharide(LPS)- induced acute pneumonia in mice.In the in vitro experiment,LPS was used to stimulate mouse alveolar macrophages(MH-S)to establish an inflammatory model,and the effect of GCP on the expression of inflammatory factors was observed.In the in vivo experiment,an LPS-in-duced acute pneumonia model in C57BL/6 mice was established to evaluate the changes in the dry-to-wet weight ratio of lung tissue,patho-logical damage,and expression levels of inflammatory factors after GCP intervention.Compared with the model group(LPS),GCP signifi-cantly reduced(P<0.001)the expression of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the LPS-induced MH-S cells in vitro.In the mouse model,compared with the model group(LPS),GCP significantly reduced(P<0.001)the wet-to-dry weight ratio of the lung tissue,improved pathological damage,and significantly inhibited(P<0.001)the expression of in-flammatory factors and the activation of the Toll-like receptor 4/nuclear factor κB(TLR4/NF-κB)signaling pathway.GCP showed good anti-inflammatory effects both in vitro and in vivo in mice,possibly by regulating the expression of the inflammatory factors to alleviate LPS- induced pulmonary inflammatory responses.GCP possessed a protective effect on LPS-induced acute pneumonia in mice.