强直性脊柱炎患者环状RNA hsa_circ_0001707的表达及意义OA

Objective To investigate the clinical value of circular RNAs(circRNAs)in the diagnosis and disease activity assessment of ankylosing spondylitis(AS)by measuring the expression levels of hsa_circ_0001707 and hsa_circ_0075522 in peripheral blood mononuclear cells(PBMCs)of AS patients.Methods A case-control study was conducted,enrolling 88 newly diagnosed AS patients and 80 healthy controls(HCs)with matched baseline characteristics.The expression of hsa_circ_0001707,hsa_circ_0075522,and HLA-B27 was detected via quantitative polymerase chain reaction(qPCR).Disease activity was evaluated using the Bath Ankylosing Spondylitis Disease Activity Index(BASDAI).Erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),and complete blood count parameters were measured using automated analyzers.Novel inflammatory indices,including the lymphocyte-to-monocyte ratio(LMR),neutrophil-to-lymphocyte ratio(NLR),platelet-to-lymphocyte ratio(PLR),derived NLR(dNLR),and systemic immune-inflammation index(SII),were calculated.Results circRNA analysis revealed significantly upregulated hsa_circ_0001707 and downregulated hsa_circ_0075522 in AS patients(both P<0.05).Receiver operating characteristic(ROC)curve analysis demonstrated that hsa_circ_0001707 exhibited moderate diagnostic efficacy for distinguishing AS from HCs(AUC=0.677,P<0.001),which significantly improved when combined with dNLR(AUC=0.835,P<0.001).In disease activity assess-ment,hsa_circ_0001707 levels were significantly higher in active AS(BASDAI≥4)than in inactive patients and correlated with CRP.Its discriminative power for active AS surpassed that of CRP(AUC:0.684 vs.0.674).Correlation analysis further supported the potential role of hsa_circ_0001707 in AS pathogenesis,showing positive associations with inflammatory markers(NLR,SII,CRP)and BASDAI(P<0.05),but negative correlations with lymphocyte percentage(L%)and LMR.In contrast,hsa_circ_0075522 showed no significant clinical correla-tions.Conclusion Hsa_circ_0001707 may serve as a promising peripheral blood biomarker for AS diagnosis and disease activity monitoring.