赤芍双菌发酵液抗氧化及抗炎舒缓功效研究OA

To enhance the antioxidant,anti-inflammatory,and soothing properties of Paeonia lactiflora(red peony root)and explore its potential applications in skincare,repair,and anti-aging,this study investigated a co-fermentation system of Paeonia lactiflora(red peony root)powder with Pseudoalteromonas sp.SE9 and Schizosaccharomyces pombe JM2-16.The antioxidant capacity of the fermentation broth was assessed using multiple free radical scavenging assays.Cytotoxicity was evaluated via the CCK-8 assay to ensure safety.The effects on skin barrier repair were examined using EdU staining for cell proliferation and scratch assays.Anti-inflammatory and soothing effects were evaluated by measuring TNF-α and IL-6 levels in an LPS-induced RAW264.7 cell model,alongside hyaluronidase inhibition assays.Results demonstrated that,within a specific concentration range,the fermentation broth exhibited significant antioxidant activity.In vitro experiments further showed potent hyaluronidase inhibition and suppression of inflammatory factor release,supporting enhanced skin repair capabilities.When incorporated into a facial cream formulation,human efficacy evaluations revealed significant reductions in redness index and skin hemoglobin content(p<0.01),confirming its antioxidant and anti-inflammatory soothing effects.