绿木霉NY45单独及与铜唑联用生物防腐应用研究OA

Wood preservation is a crucial approach to prevent infestation by decay fungi and to extend the service life of wood materials.This study aimed to investigate the potential of using the antagonistic microorganism Trichoderma virens(strain NY45),either alone or in combination with the chemical preservative copper azole,for bio-preservation.The goal was to develop a more environmentally friendly treatment method by reducing the required amount of copper-based preservatives.In this study,Populus tomentosa and Pinus radiata specimens were treated with the spore solution of T.virens NY45 as a treatment agent,and the effect and process of individual treatment conditions and joint application with copper-based preservative copper azole were explored through the laboratory decay resistance tests and combination tests when T.virens NY45 was as a bio-preservative fungi.The experiment was conducted by setting different time intervals(the same day,after 7 d,after 15 d,after 30 d and after 60 d)from infection of the specimens to the soaking treatment of T.virens NY45 spore solution to explore the best treatment time.Another group of spore solution of T.virens NY45 was set four number concentration gradients(1×104-2×104,1×105-2×105,1×106-2×106,and 1×107-2×107 germ/mL)to explore the optimum number concentration.Furthermore,the spore solution of T.virens NY45 and the retention of 0.01,0.05 and 0.10 kg/m3 of copper azole were combined to treat specimens to test the decay resistance.The results showed that the spore solution treatment of T.virens NY45 had a significant protective effect on wood specimens when applied before or within one week prior to infestation by fungus.Under these conditions,the specimens achieved strong decay resistance,corresponding to Class I.The optimal number concentration of the spore solution was in the range of 1×106-2×107 germ/mL.It was found that the decay resistance of Populus tomentosa and Pinus radiata treated with T.virens NY45 in combination with varying retention levels of copper azole was enhanced compared to treatment with the same number concentration of copper azole alone.The combined treatment was particularly effective when the copper azole retention was≥0.05 kg/m3.In this experiment,the optimum time and number concentration of T.virens NY45 spore solution were determined by anti-corrosion effect tests,the results highlighted the efficacy of combining NY45 with copper-based preservatives,suggesting a promising approach to minimize copper usage and cut preservation costs.This study has established a foundation for integrating T.virens NY45 as a biological preservative in wood protection,advancing the development of eco-friendly wood protection solutions.