人偏肺病毒快速检测方法的建立及应用OA

Currently,the detection of human metapneumovirus(hMPV)primarily relies on methods such as viral nucleic acid testing,viral antigen detection,viral culture and isolation,and serological testing.These methods are often complex,time-consuming,costly,and highly dependent on specialized equipment.In contrast,lateral flow immunochromatography offers advantages such as low cost,high specificity,strong stability,rapid detection,and the ability to be performed without specialized personnel or equipment,providing a new approach for hMPV detection.This study utilized monoclonal antibody technology to obtain specific monoclonal antibodies targeting the N protein of hMPV.The antibodies were used to label colloidal gold and applied for the detection of human metapneumovirus N protein based on a double-antibody sandwich immunochromatographic assay.The established rapid detection method for human metapneumovirus N protein achieved a detectio limit of 0.1 μg/L,demonstrating high sensitivity,accuracy,and specificity.This method can be used for rapid on-site detection of metapneumovirus.