基于便携式荧光试纸条的水体抗生素比率荧光检测方法研究OA
Research on a Ratiometric Fluorescence Detection Method for Antibiotics in Water Based on Portable Fluorescent Test Strips
通过开发一种高灵敏、高选择性的新方法,以应对水环境中抗生素污染对生态环境和人类健康造成的危害.采用一步法合成沸石咪唑酯-8(ZIF-8)材料,通过FT-IR、XRD、SEM、TEM、XPS等多种表征技术对其结构进行系统分析,构建基于比率荧光的左氧氟沙星(LFX)检测传感器.在ZIF-8浓度为25 μg/mL、pH为7.0、反应时间为5 min的最佳反应条件下,所制备传感器对LFX的线性检测范围为1～80 μmol/L,检出限达0.29 μmol/L;实际水样检测回收率98.27％～101.90％,RSD≤2.99％,与HPLC结果吻合良好;开发的智能手机便携式试纸传感器可实现10～100 μmol/L范围内的实时检测.该研究为ZIF-8基荧光传感器的设计提供了新思路,建立了环境水体中痕量抗生素污染物的快速检测新方法.
This study aims to develop a novel,highly sensitive and selective method to address the ecological and human health risks posed by antibiotic pollution in aquatic environments.Zeolitic imidazolate framework-8(ZIF-8)was synthesized via a one-step method and systematically characterized using FT-IR,XRD,SEM,TEM,and XPS.A ratiometric fluorescence sensor was then constructed for the detection of levofloxacin(LFX).Under optimal conditions(ZIF-8 concentration:25 μg/mL,pH 7.0,reaction time:5 min),the sensor exhibited a linear detection range of 1～80 μmol/L for LFX,with a detection limit of 0.29 μmol/L.The recovery rate in real water samples was from 98.27％to 101.9％,with RSD≤2.99％,showing good agreement with HPLC results.Additionally,a smartphone-based portable test strip sensor was developed,enabling real-time detection in the range of 10～100μmol/L.This study provides a new approach for designing ZIF-8-based fluorescence sensors and establishes a rapid detection method for trace antibiotic pollutants in environmental water.
李凤兰;黄碧妃;林泽彪;黄文杰
福建警察学院刑事科学技术系,福建 福州 350007福建警察学院刑事科学技术系,福建 福州 350007福建警察学院刑事科学技术系,福建 福州 350007福建警察学院侦查系,福建 福州 350007
化学化工
ZIF-8比率型荧光传感器左氧氟沙星荧光试纸
ZIF-8ratiometricfluorescence sensorlevofloxacin(LFX)fluorescent test strip
《化学试剂》 2026 (1)
36-42,7
福建省自然科学基金项目(2024J08115)福建省社会科学基金博士扶持项目(FJ2023BF079).
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