甘草对附子中3种生物碱的体外稳定性影响研究OA

Objective To investigate the effects of licorice and its different polar parts on the in vitro stabil-ity of three alkaloids(aconitine,hypaconitine,benzoylmesaconine)in Aconiti Radix Lateralis Praeparata under different environmental conditions.Methods Ultra performance liquid chromatography was used to analyze the content changes of three alkaloids under different conditions(pH,temperature),and the chemical kinetic method was applied to fit the degradation kinetics and calculate the relevant kinetic parameters.Results The three alkaloids degraded significantly under alkaline conditions(pH 8.3)and conformed to the first-order ki-netic model.The licorice extract improved the stability of the three alkaloids to a certain extent,and had the most significant effect on the stability of hypaconitine.The degradation of three alkaloids under high temperature conditions(60℃,70℃and 90℃)conformed to the first-order kinetics,and the licorice extract significantly delayed the degradation rate.The licorice extract and its different polar components could promote the stability of aconitine and hypaconitine at pH 8.3,but had no significant effect on benzoylmesaconine.Conclusion Lico-rice and its different polar parts can affect the stability of the three main alkaloids in Aconiti Radix Lateralis Pra-eparata to varying degrees,among which licorice extract showed the most prominent effect.The scientific conno-tation of the compatibility of licorice and Aconiti Radix Lateralis Praeparata is interpreted from the perspective of stability.