基于高分辨液相色谱与质谱联用系统分析银鲳肌肉脂质组成OA
Analysis of lipid composition in Pampus argenteus muscle using high-resolution liquid chromatography-mass spectrometry
[目的]研究银鲳(Pampus argenteus)肌肉的脂质组成,为银鲳在食品加工和功能产品开发等领域的应用提供科学依据.[方法]将野生银鲳肌肉样品冷冻干燥后,经液氮研磨成粉,用甲醇、氯仿萃取后提取肌肉样品的总脂质;利用高分辨液相色谱与质谱联用系统,对银鲳肌肉样品的总脂质成分进行定性和定量分析.[结果]银鲳肌肉样品中共鉴定到17 大类共 447 种脂质,包括鞘脂(SL)4 类,分别为鞘氨醇(So)、神经酰胺(Cer)、单糖鞘脂(CerG1)和鞘磷脂(SM);中性甘油脂 2 类,分别为甘油二酯(DAG)和甘油三酯(TAG);磷脂 11 类,分别为溶血磷脂酰胆碱(LPC)、磷脂酰胆碱(PC)、溶血磷脂酰乙醇胺(LPE)、磷脂酰乙醇胺(PE)、溶血磷脂酰丝氨酸(LPS)、磷脂酰丝氨酸(PS)、溶血磷脂酰甘油(LPG)、磷脂酰甘油(PG)、溶血磷脂酰肌醇(LPI)、磷脂酰肌醇(PI)和磷脂酸(PA).其中,中性甘油脂和磷脂含量分别占总脂质含量的 54.93%和43.82%;中性甘油酯中,甘油三酯(TAG)的种类和含量最多,占总脂质含量的 38.37%;而磷脂中PC含量最多,PE含量次之,分别占总脂质含量的 25.32%和 11.48%.在银鲳肌肉样品中共检测出 32 种脂肪酸,包括 11 种饱和脂肪酸(SFA)、8 种单不饱和脂肪酸(MUFA)和 13 种多不饱和脂肪酸(PUFA),其中PUFA含量略高于SFA和MUFA.银鲳肌肉样品中主要的脂肪酸组成为棕榈酸(C16:0)、油酸(C18:1)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA).[结论]本研究利用高分辨液相色谱与质谱联用系统分析了银鲳肌肉脂质组成,发现银鲳脂质组成多样,且富含多不饱和脂肪酸 DHA 和 EPA,符合"蓝色粮仓"对营养健康水产品的定位.
[Objective]This study aims to elucidate the lipid composition of silver pomfret(Pampus argenteus)muscle,establishing a scientific foundation for its potential applications in food processing and functional food development.[Methods]Wild P.argenteus samples were freeze-dried and ground into powder using liquid ni-trogen.Total lipids were extracted from the muscle samples using methanol and chloroform.The lipid profile in muscle of P.argenteus was explored by high-resolution liquid chromatography-mass spectrometry equipped with a HESI-Ⅱ probe.[Results]A total of 447 lipids belonging to 17 major classes were identified in P.ar-genteus.These included 4 classes of sphingolipids:sphingosine(So),ceramide(Cer),monoglycosylceramide(CerG1),and sphingomyelin(SM);2 classes of neutral glycerolipids:diacylglycerol(DAG)and triacylglycerol(TAG);11 classes of phospholipids:lysophosphatidylcholine(LPC),phosphatidylcholine(PC),lysophos-phatidy-lethanolamine(LPE),phosphatidylethanolamine(PE),lysophosphatidylserine(LPS),phosphatidylser-ine(PS),lysophosphatidylglycerol(LPG),phosphatidylglycerol(PG),lysophosphatidylinositol(LPI),phos-phatidylinositol(PI),and phosphatidic acid(PA).Quantitative analysis demonstrated that neutral glycerolipids(54.93%)and phospholipids(43.82%)constituted the predominant lipid fractions.Triglycerides(TAG)repres-ented the most abundant lipid species(38.37%of total lipids),while phosphatidylcholine(25.32%)and phos-phatidylethanolamine(11.48%)emerged as the major phospholipid components.Fatty acid profiling identified 32 molecular species,comprising 11 saturated fatty acids(SFA),8 monounsaturated fatty acids(MUFA),and 13 polyunsaturated fatty acids(PUFA),with PUFA exhibiting marginally higher abundance than SFA and MUFA.The major fatty acids in P.argenteus muscle were palmitic acid(C16:0),oleic acid(C18:1),eicosapentaenoic acid(EPA),and docosahexaenoic acid(DHA).[Conclution]High-resolution liquid chromatography-mass spectrometry analysis demonstrated that P.argenteus muscle contains a diverse array of lipids,with notably high levels of DHA and EPA,supporting its classification as a nutritious seafood under the"Blue Granary"framework.
牟桐;黄莉莉;朱竹君;李艳荣;徐继林
宁波海洋研究院,浙江 宁波 315832宁波海洋研究院,浙江 宁波 315832宁波海洋研究院,浙江 宁波 315832宁波海洋研究院,浙江 宁波 315832宁波大学海洋学院,浙江 宁波 315211
农业科技
银鲳脂质脂肪酸高分辨液相色谱与质谱联用系统
Pampus argenteuslipidsfatty acidshigh-resolution liquid chromatography-mass spectrometry
《渔业研究》 2026 (1)
85-95,11
国家现代农业产业技术体系建设专项资金项目(CARS-49)浙江省自然科学基金项目(LTGC24D060001)宁波市科技攻关项目(2024Z276)
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