培养条件对微拟球藻FACHB-2541生长和EPA合成的影响OA

[Background]Nannochloropsis is a typical oil-producing microalga characterized by rapid growth and high eicosapentaenoic acid(EPA)content.However,its large-scale application is currently limited by low biomass yield and insufficient EPA accumulation,highlighting the need to optimize cultivation conditions to improve productivity.[Objective]This study aims to optimize the cultivation conditions of N.gaditana FACHB-2541 to enhance its eicosapentaenoic acid(EPA)production,providing theoretical support and technic-al guidance for its large-scale,high-value applications in aquaculture,food,and pharmaceutical industries.[Methods]Using f/2 medium as the initial medium,the effects of different nitrogen sources[sodium nitrate(the control group),urea,ammonium chloride,and ammonium acetate],nitrogen concentrations(50,100,150 and 200 mg/L),and light intensities[75,150 and 200 µmol/(m2·s)]on the growth,lipid accumulation,and EPA biosynthesis of N.gaditana FACHB-2541 were investigated.A single-factor experimental design was em-ployed,and multiple parameters including biomass,lipid content,and EPA yield were evaluated to determine the optimal cultivation conditions.[Results]The results showed that the set levels of cultivation parameters(ni-trogen sources,nitrogen concentration and light intensity)had significant effects on the growth performance and EPA production of N.gaditana FACHB-2541.Among the tested nitrogen sources,ammonium acetate was more efficiently utilized by N.gaditana FACHB-2541,resulting in faster growth and higher EPA accumulation.At a concentration of 150 mg/L nitrogen(ammonium acetate),N.gaditana FACHB-2541 achieved the highest bio-mass and EPA yield.Moreover,a light intensity of 75 µmol/(m2·s)was more favorable for growth and EPA pro-duction compared to higher intensities[150 and 200 µmol/(m2·s)].Under the optimized conditions(ammoni-um acetate as the nitrogen source at 150 mg/L;light intensity[75 µmol/(m2·s)],the microalga exhibited the maximum biomass of 2.10 g/L and EPA yield of 156.22 mg/L;which were increased by 107.92%and 73.31%compared to before optimization.[Conclusion]N.gaditana FACHB-2541 under the optimized cultivation con-ditions was the potential candidate to exploit high-valued EPA production.These studies provide a solid theoret-ical basis and practical parameters for enhancing the production efficiency of N.gaditana,especially for applic-ations requiring high-value EPA output.The optimized cultivation strategy proposed here offers operational simplicity and scalability,making it promising for industrial-scale cultivation in sectors such as aquaculture,nutraceuticals,and functional food development.