电针胃合募配穴通过PI3K/AKT/mTOR通路改善抑郁模型小鼠胃功能障碍OA

Objective To investigate whether electroacupuncture at stomach He points and Front-Mu points improves gastric dysfunction in mice with depression by regulating the phosphoinositide-3 kinase(PI3K)/protein kinase-B(AKT)/mammalian target of rapamycin(mTOR)signaling pathway.Methods A total of 30 male C57BL/6 mice were randomly divided into blank group,model group,and electroacupuncture group,with 10 mice in each group,and chronic unpredictable mild stress(CUMS)was used to establish a mouse model of depression.The mice in the electroacupuncture group were given electroacupuncture at Zusanli and Zhongwan twice a day for 7 consecutive days,while those in the blank group and the model group were not given any intervention.The tail suspension test,the forced swimming test,the open field test,and the elevated plus maze test were used to observe behavioral changes;body weight,food intake,gastric emptying rate,and the amplitude of gastric motility were measured;HE staining was used to observe the histomorphology of gastric antrum,and TUNEL staining was used to observe the apoptosis of gastric antrum smooth muscle cells;Western blot was used to measure the expression levels of the proteins as-sociated with the PI3K/AKT/mTOR signaling pathway in gastric antrum smooth muscle.Results Electroacupuncture inter-vention significantly reduced immobility time in the tail suspension test and the forced swimming test(P＜0.05)and increased the number of entries into the central zone and the total movement distance in the open field test(P＜0.05).It also increased the percentage of open-arm dwell time(P＜0.05)and the percentage of movement distance into the open arm(P＜0.05),as well as body weight,food intake,gastric emptying rate,and the amplitude of gastric motility(P＜0.05).In addition,it re-duced the apoptosis rate of gastric antrum smooth muscle cells(P＜0.05)and increased the expression levels of the proteins as-sociated with the PI3K/AKT/mTOR signaling pathway(P＜0.05).HE staining showed that there were no obvious pathologi-cal changes in the gastric antrum in the electroacupuncture group.Conclusion Electroacupuncture may improve gastric dys-function in mice with depression by activating the PI3K/AKT/mTOR signaling pathway and inhibiting the apoptosis of gastric antrum smooth muscle cells.