基于间接竞争性酶联免疫吸附法研究枳实炭碳点对芍药苷的药代动力学影响OA

Objective:To investigate the pharmacokinetic effects of Aurantii Fructus Carbon Quantum Dots(AFIC-CDs)on paeoniflorin(PF),the main active component of Paeonia.Methods:Male Kunming mice were randomly divided into three groups:high-dose AFIC-CDs(120 mg·mL-1)and PF co-administration group(H),low-dose AFIC-CDs(60 mg·mL-1)and PF co-administration group(L),and PF alone(2.0 mg·mL-1)group(S).Mice were orally administered mixed solutions of high or low-dose AFIC-CDs with the same dose of PF,or PF alone.Paeoniflorin levels in the mouse blood were determined by indirect competitive enzyme-linked immunosorbent assay(icELISA)using anti-PF monoclonal antibodies,and pharmacokinetic parameters of PF were compared among the groups.Results:Co-administration of AFIC-CDs with PF significantly increased PF blood concentration and the area under the blood concentration-time curve(AUC-t)(P<0.05,P<0.01),with increases observed as the AFIC-CDs concentration increased.Compared to PF administration alone,the Cmax of PF in the high-dose and low-dose AFIC-CDs+PF groups increased from(695.43±229.73)ng·mL-1 to(1 615.31±245.16)ng·mL-1 and(973.11±155.90)ng·mL-1,respectively.The AUC-t increased from(90181.22±20926.42)ng·min·mL-1 to(257 510.74±26 729.64)ng·min·mL-1 and(116 654.67±11 770.02)ng·min·mL-1,respectively.Conclusion:AFIC-CDs can enhance the bioavailability of PF,providing scientific evidence for clinical co-medication to improve therapeutic efficacy.