大黄灵仙方对胆囊结石豚鼠模型肠道菌群的影响OA

Objective:To investigate the impact of Dahuang Lingxian Formula(Rheum Officinale and Radix Clematidis Formula,DHLX)on the intestinal microbiota's structure and function in guinea pigs suffering from gallbladder stones.Methods:Fifty guinea pigs were randomly assigned to a blank group,a model group,and a traditional Chinese medicine(TCM)group.The model group consumed a high-fat diet to induce gallbladder stones,while the TCM group received DHLX for 8 weeks alongside the same high-fat diet.Colon fecal samples were collected at the 8th week and subjected to 16S rDNA sequencing for analyzing intestinal flora composition,diversity,and functional predictions.Results:①The OTU count was 4623 in the normal group,3970 in the model group,and 4335 in the TCM group;474 OTUs were common to all three groups.②Major dominant phyla encompassed Bacteroidetes,Firmicutes,Proteobacteria,Verrucomicrobia,and Spirochaetes.In the model group,Bacteroidetes and Verrucomicrobia increased,while Firmicutes and Spirochaetes decreased.Conversely,in the TCM group,Bacteroidetes,Firmicutes,and Verrucomicrobia decreased,while Proteobacteria and Spirochaetes increased.③The intestinal flora diversity index(Ace,Chao1,Shannon,Simpson)showed no significant difference between groups.④The intestinal flora distribution of the model group significantly diverged from the normal group,with partial overlap observed between the TCM and model groups.⑤In the blank group,Ruminococcus,Rikenellaceae,and other genera were highly expressed;Paludibacteraceae and Verrucomicrobia were prominent in the model group;Alphaproteobacteria and Desulfovibrio were highly expressed in the TCM group.⑥The intestinal microbiota of the blank group exhibited anaerobic and biofilm synthesis traits.In the model group,Gram-negative bacteria increased,while mobile genetic elements and biofilm synthesis decreased.However,in the TCM group,the characteristics of Gram-negative bacteria and biofilm synthesis were restored.Conclusion:The intestinal microbiota structure undergoes significant changes during gallbladder stone formation,and DHLX effectively regulates the flora,restoring it to a normal steady state.