3种检测方法比较不同基原泽泻的体外降脂活性OA

Objective To compare the differences in in vitro lipid-lowering activity of Alismatis Rhizoma from different origins and regions,providing a scientific basis for the clinical use of Alisma in treating hyperlipidemia and the development of related products.Methods The in vitro lipid-lowering activity of 30 batches of Alismatis Rhizoma samples was evaluated using three assays:sodium taurocholate binding rate,pancreatic lipase inhibition rate,and cholesterol esterase inhibition rate.Chemical pattern recognition was performed using hierarchical cluster analysis(HCA)and partial least squares-discriminant analysis(PLS-DA).Results The lipid-lowering activity of Alismatis Rhizoma exhibited significant dependence on origins and regions.HCA and PLS-DA analyses demonstrated distinct clustering patterns among samples of different origins.The established origin-discrimination model demonstrated good explanatory power and predictive ability(R2x=0.75,Q2=0.867),while the regions model displayed complete explanatory power(R2x=1)and moderate predictive ability(Q2=0.555).The Alisma orientale group generally exhibited higher cholesterol esterase inhibition rates than the Alisma plantago-aquatica group.Conclusion Alismatis Rhizoma may exerts its lipid-lowering effects primarily through cholesterol esterase inhibition,with Alisma orientale demonstrating superior activity.These findings provide experimental evidence for the selection of genuine regional medicinal materials and the development of lipid-lowering products.