单细胞测序技术在畜禽遗传育种中的应用研究进展OA

Cells are the fundamental units that make up living organisms,possessing unique developmental trajectories and molecular features.Single cell sequencing technology,as an efficient method for analyzing genetic information at the cellular level,can sequence the genome,transcriptome,proteome,metabolome,and epigenetics,and support integrated analysis strategies such as multi-omics sequencing to accurately capture the molecular characteristics of individual cells.This technology breaks through the limitations of traditional population cell research,and has become a powerful tool for analyzing the molecular regulatory mechanisms and cell fate transition trajectories of animal cells,providing a new perspective for animal genetics breeding.The artical focuses on five single-cell sequencing technologies—Single-cell RNA sequencing,single-cell DNA sequencing,single-cell epigenome sequencing,single-cell proteomics sequencing,and single-cell metabolomics sequencing,systematically elaborates on the developmental history and underlying principles,and discusses in depth the application cases of single-cell sequencing technology in livestock and poultry genetic breeding,aiming to provide a theoretical reference for subsequent research in this field.