旋幽夜蛾成虫行为昼夜节律OA

[Objective]To investigate the circadian rhythm of adult activity in Discestra trifolii,[Methods]five behaviors of paired adults,including moving,feeding,mating,oviposition and resting were observed for 6 consecutive days in the laboratory,and the observed data were analyzed.[Results]The results showed that within 6 days after pairing,the percentages of moving,feeding and resting behaviors were different among Discestra trifolii of different day-old ages and between females and males within same age,but these differences were not significant.The percentage of mating behavior was highest on the third day,and oviposition occurred from the second day after pairing;In 2-5.5 h of scotophase,females had higher percentages of moving behavior than males,and there was a significant difference at 2.5 h of scotophase.After 6 h of scotophase,males had higher percentages of moving behavior than females,but there was no significant difference.The feeding behavior of females had two peaks at 0.5 h and 7 h of scotophase,and significantly higher than males at 0.5 h of scotophase.The feeding behavior of males had a peak at 10.5 h of scotophase,and significantly higher than females.The mating behavior mainly occurred from 7 h of scotophase to 1 h of photophase,and the peak occurred at 10 h of scotophase.The oviposition behavior of females occurred from 0 h to 10 h of scotophase,and the scotophase from 0 h to 2 h was the main period of oviposition behavior.The largest percentage of diurnal time distribution in adult behaviors was resting behavior,which was significantly higher than other behaviors.There was no significant difference in the percentage of diurnal time distribution for the same behavior between females and males.The percentages of diurnal time distribution of adult behaviors had significant difference between photophase and scotophase.In photophase,the females and males were basically in the resting state,while other behaviors mainly occurred in scotophase,but rarely occurred in photophase.Except for the moving and feeding behaviors of females,all other behaviors had significant differences in different periods of scotophase.[Conclusion]The results showed that after pairing,the behaviors of adults were influenced by the light-dark cycle,sex and day-old age,and the behavior rhythm was obvious.