基于Touchdown程序的甜菜SRAP核心引物的筛选OA
Screening of SRAP Core Primers for Sugar Beet Based on Touchdown Program
[目的]旨在筛选出高多态性、高稳定性的SRAP核心引物用于甜菜遗传多样性的鉴定.[方法]利用Touchdown程序对SRAP引物组合进行扩增,用 8%的非变性聚丙烯酰胺凝胶电泳对扩增条带进行分离,基于扩增结果进行数据分析筛选出符合要求的引物.[结果]从 24 种上游引物与21 种下游引物的504 对组合中筛选出 43 对高多态性、高稳定性的引物组合,其中 40 对引物组合的多态性信息量(PIC)值大于0.75,24 对引物组合PIC值大于0.8,4 对引物组合PIC值大于0.9.多态性信息量范围在0.657～0.953,引物对的平均PIC值为0.812.[结论]SRAP核心引物的筛选为甜菜的遗传多样性鉴定以及进一步杂交育种的亲本选择、分子标记辅助育种等提供理论支撑.
[Objective]The main objective is to select SRAP core primers with high polymorphism and high stability for the identification of genetic diversity in sugar beet.[Methods]Amplification was performed using SRAP primer combinations under a Touchdown program.The products were separated by 8%non-denaturing polyacrylamide gel electrophoresis,and primers meeting the criteria were selected based on data analysis of the amplification results.[Results]From 504 pairs consisting of 24 upstream primers and 21 downstream primers,43 pairs of primer combinations with high polymorphism and stability were identified,of which 40 pairs of primer combinations had a polymorphism information content(PIC)value greater than 0.75,24 pairs of primer combinations had a PIC value greater than 0.8,and 4 pairs of primer combinations had a PIC value greater than 0.9.The PIC range was 0.657 to 0.953,and the average PIC value of primer pairs was 0.812.[Conclusion]The screening of SRAP core primers provides theoretical support for the identification of genetic diversity in sugar beet,as well as for the selection of parents for further cross-breeding and molecular marker-assisted breeding.
张程伟;刘佳俊;阚文亮;宋玥;吴则东
黑龙江大学现代农业与生态环境学院,哈尔滨 150080黑龙江大学现代农业与生态环境学院,哈尔滨 150080黑龙江省农垦科学院经济作物研究所,哈尔滨 150000黑龙江大学现代农业与生态环境学院,哈尔滨 150080黑龙江大学现代农业与生态环境学院,哈尔滨 150080
农业科技
甜菜SRAP引物分子标记核心引物
sugar beetSRAP primersmolecular markerscore primers
《中国糖料》 2026 (1)
40-46,7
大学生创新创业训练计划项目"适宜于甜菜遗传多样性鉴定的SRAP核心引物的筛选及其应用"(S202410212150)国家现代农业产业技术体系(糖料)项目(CARS-17)资助.
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