金匮肾气丸和六味地黄丸对正常小鼠生殖能力及其脑功能影响的比较OA

Objective:To explore the effects of Jingui Shenqiwan(JSW)and Liuwei Dihuangwan(LDW)on the reproductive ability and brain function of normal mice and compare the actions of the two medications.Methods:Seven groups of female and male mice were divided at a ratio of 2∶1.Except for the control group,the other six groups were as follows:a group of both males and females receiving JSW(3.0 g·kg-1),a group of both males and females receiving LDW(4.5 g·kg-1),a group of males receiving water and females receiving JSW,a group of males receiving water while females receiving LDW,a group of females receiving water while males receiving JSW,and a group of females receiving water while males receiving LDW.Each group was administered the drug for 14 days and then caged together at a 2∶1(female∶male)ratio to detect the number of pregnant mice and calculate the pregnancy rate.Pregnant mice continued receiving the drug until they naturally gave birth,which was followed by the observation of newborn mice,calculation of their average number,and the measurement of the offspring's preference for sugar water and neonatal recognition index.At the end of the experiment,the weights of the thymus and spleen were measured to calculate the organ coefficients,and mRNA or protein expression was analyzed in the brain and testes or ovaries.A 1%sucrose solution was used to examine the euphoria of their brain reward systems,while novel object recognition test(NOR)was applied to assess their memory capabilities.mRNA expression was detected using real-time quantitative polymerase chain reaction(Real-time PCR)assay,and protein expression was analyzed with Western blot.Results:Compared with the control group,oral administration of JSW to both male and female mice for 14 days significantly increased the pregnancy rate of female mice on day 2 after being caged together(P<0.05),while LDW showed a trend but no statistical significance.Additionally,compared with the control group,JSW could upregulate the gene expression of gonadotropin-releasing hormone(GnRH)in the thalamus,as well as reproductive stem cell factor(SCF)and tyrosine kinase receptor(c-Kit)in the testes and reproductive stem cell marker mouse vasa homologue(MVH)in the ovaries,upregulate the expression of proteins influencing neuronal functional activity,such as brain-derived neurotrophic factor(BDNF),in hippocampal neurons(P<0.05),and enhance sucrose preference in male mice(P<0.05).Compared with the control group,JSW significantly increased sucrose preference and novel object recognition index in offspring mice(P<0.05),which was related to the upregulation of hippocampal dopamine D1 receptor(D1R)and N-methyl-D-aspartate receptor(Nmdar)gene expression.Compared with the control group,both JSW and LDW could upregulate the protein expression of glucocorticoid receptor(GR),BDNF,and tyrosine kinase receptor B(TrkB)in the hippocampus of offspring mice(P<0.05).Conclusion:JSW significantly enhances the reproductive ability of normal mice,which is not only related to the release of gonadotropin but also associated with its regulation of brain function.Additionally,JSW has a certain regulatory effect on the brain function of the offspring mice.