野生平菇产纤维素酶发酵条件的优化OA

Cellulose was a renewable resource with a wide range of sources,but its utilization rate was low.Some saprophytic microorganisms such as shiitake mushrooms can produce cellulases,lacases,etc.to degrade them.The experiment selected a wild strain of Pleurotus ostreatus preserved in the laboratory to explore the effects of medium composition and fermentation conditions on the cellulase production capacity of Pleurotus ostreatus through single factor experiments.Then,three influential factors,temperature,phosphate concentration and fermentation days were selected,and response surface methodology was used to evaluate cellulase activity.The results showed that the optimum fermentation conditions were as follows:temperature 20℃,phosphate concentration 0.2%,fermentation time 5 days,inoculation amount 10%,rotating speed 150 r/min,liquid volume 50 mL and pH 7.Under these conditions,the activity of cellulase produced by Pleurotus ostreatus was as high as 12.6375 U/mL.The results of this experiment could provide some theoretical basis for the subsequent study on the ability of Pleurotus ostreatus to produce cellulase.