隔山牛皮消中2个新化学成分研究OA

Objective To investigate the chemical constituents and their anti-inflammatory activity of Cynanchum wilfordii.Methods Various chromatographic techniques including silica gel,ODS,and Sephadex LH-20 column chromatography were employed for the isolation and purification of compounds.Their structures were elucidated through comprehensive spectroscopic analysis including HR-ESI-MS,ESI-MS,1H-NMR,13C-NMR,HSQC,and HMBC.The anti-inflammatory activity of the compounds was evaluated using the Griess method.Results Two compounds were isolated and identified as(7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside(1)and 3β-[(O-β-D-glucopyranosyl)-oxy]-8β,14β-trihydroxycarda-5,20-(22)-dienolide(2).Compounds 1 and 2 inhibited NO production by 55.16%and 15.34%,respectively,with a median inhibition concentration(IC50)of 41.37 μmol/L for compound 1.Conclusion Compounds 1 and 2 are both new compounds,belonging to lignan and cardenolide types,respectively.They have been assigned the names cynawilforin A(1)and wilforcardenoside A(2).In vitro anti-inflammatory assays indicated that compound 1 exhibits certain anti-inflammatory activity.