黑木耳多糖提取工艺优化及其体外抗氧化与抗糖活性研究OA

This study employed enzymatic hydrolysis to extract polysaccharides from Auricu-laria auricula.The extraction conditions for the polysaccharides were optimized through sin-gle-factor experiments and response surface methodology,using polysaccharide yield as the primary indicator.Two polysaccharide components,designated AAP-1 and AAP-2,were isolated via DEAE cellulose column chromatography,and their total sugar,protein,uronic acid,and total phe-nol contents were quantified.The antioxidant capacities of AAP-1 and AAP-2 were evaluated based on their scavenging rates of DPPH radicals,hydroxyl radicals,and superoxide anions.Additionally,the hypoglycemic effects of AAP-1 and AAP-2 were assessed through their inhibition rates on α-am-ylase and α-glucosidase.The results revealed that the optimal extraction conditions for polysaccha-rides from Auricularia auricula are as follows:enzymatic hydrolysis pH of 5,temperature of 50 ℃,duration of 90 minutes,and enzyme concentration of 40 mg/g.Compared to crude polysaccharides,the total sugar content in the eluted AAP-1 and AAP-2 significantly increased,while impurities such as protein,uronic acid,and total phenols were markedly reduced.Specifically,at a concentration of 0.8 mg/mL,AAP-1 and AAP-2 demonstrated DPPH radical scavenging rates of 49.05％and 62.69％,hydroxyl radical scavenging rates of 34.04％and 45.02％,and superoxide anion scaven-ging rates of 38.62％and 39.98％,respectively.Furthermore,at a concentration of 1.0 mg/mL,the inhibition rates of AAP-1 and AAP-2 on α-glucosidase were 72.24％and 87.10％,respectively,while their inhibition rates on α-amylase were 80.51％and 89.23％,respectively.These findings in-dicate that both AAP-1 and AAP-2 possess notable antioxidant and hypoglycemic properties,with AAP-2 displaying superior in vitro activity,thereby providing a theoretical foundation for the further development of polysaccharides derived from Auricularia auricula.