10个意大利杨树新无性系的抗寒性评价OA

In order to evaluate the cold resistance of 10 Populus clones introduced from Italy,new Populus clones suitable for cultivation in the"Three North"cold region were selected,and the annual dormant branches of 10 clones were taken as the test objects.The samples were treated with artificial gradient cool-ing method(room temperature(CK),-10,-15,-20,-25,-30℃)for 24 hours,and the changes of rel-ative conductivity,superoxide dismutase(SOD)activity,peroxidase(POD)activity,catalase(CAT)activi-ty,malondialdehyde(MDA)content and soluble protein(SP)content of the samples were measured.Based on the membership function method and correlation analysis evaluation index,the cold resistance of 10 Populus clones was comprehensively evaluated.Results showed that there were significant differences in the six cold resistance indexes of each clone.Among them,the P.nigra clone Qg had excellent comprehen-sive performance in various indexes and the strongest cold resistance.Soluble protein(SP)and malondial-dehyde(MDA)were highly positively correlated with the activities of protective enzymes.The cold resist-ance of each clone is ranked according to the membership function value as follows:Qg＞Ta＞A54＞A50＞Ti＞Pa＞A23＞A46＞La＞A39.Clones Qg and A54 are selected cold-resistant cultivars of P.nigra and P.alba,respectively,and are recommended for preliminary regional promotion.