骨髓间充质干细胞外泌体缓解小鼠哮喘模型的功能和机制OA

Objective The aim of this study was to investigate the effects and mechanisms of bone marrow mesenchymal stem cell exosomes on the mouse asthma models.Methods C57/6J mice were divided into control group,model group,exosome(5,10,and 20 μg),and exosome(20 μg)+RS09 TFA group.After administra-tion of the respective treatment,airway resistance was measured in each group of mice.Hematoxylin and eosin(HE)staining was used to detect airway damage in mice.Enzyme-linked immunosorbent assay(ELISA)was applied to detect the concentrations of inflammatory factors interleukin-4(IL-4),interleukin-5(IL-5),and inter-leukin-13(IL-13)in the alveolar fluid of mice.The colorimetric method was used to determine the concentrations of malondialdehyde(MDA),catalase(CAT),and superoxide dismutase(SOD)in the alveolar fluid.Western blot was used to detect the expression of Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),and nuclear factor κB(NF-κB)in the lung tissues of mice.Results Compared with the model group,the airway resistance in the exosome 5,10,20 μg groups decreased significantly(P<0.05);the concentrations of IL-4,IL-5,IL-13,and MDA in the alveolar fluid of mice decreased significantly(P<0.05);while the concentrations of CAT and SOD increased significantly(P<0.05);additionally,the expression of TLR4,MyD88,and NF-κB were all significantly downregulated(P<0.05).Compared with the exosome 20 μg group,the airway resistance in the exosome 20 μg+RS09 TFA group increased significantly(P<0.05),the concentrations of IL-4,IL-5,IL-13,and MDA in the alveolar fluid of mice increased significantly(P<0.05),while the concentrations of CAT and SOD decreased significantly(P<0.05),the expression of TLR4,MyD88,and NF-κB were all significantly upregulated(P<0.05).Conclusion Bone marrow mesenchymal stem cell exosomes can alleviate mouse asthma by inhibiting the TLR4/MyD88/NF-κB signaling pathway.