余甘子提取物通过蛋白激酶B/哺乳动物雷帕霉素靶蛋白通路对肺癌细胞A549恶性生物学行为的影响OA

Objective:To investigate the effect of Phyllanthus emblica extract on the malignant biological behav-ior of lung cancer cell A549 through protein kinase B/mammalian target of rapamycin(Akt/mTOR)pathway.Meth-ods:Human non-small cell lung cancer A549 cells were selected and divided into control group(blank untreated),low dose group(50 μg/ml),medium dose group(100 μg/ml)and high dose group(200 μg/ml).CCK8 method and flow cytometry were used to detect the survival and apoptosis rate of A549 cells in each group.Transwell chamber assay and cell scratch test were used to detect the invasion and migration ability of each group.Western blot was used to detect the protein expression of Akt,p-Akt,mTOR and p-mTOR in each group.Lung cancer A549 cells were collect-ed from each group to establish a mouse model of transplanted tumor.The growth of transplanted tumor and survival of mice in each group were recorded.Results:Compared with the control group,the tumor volume and weight of the mice in the low-,medium-,and high-dose Phyllanthus emblica extract groups were decreased in a dose-dependent manner(all P＜0.05).The cell viability,invasion and migration abilities and the expressions of p-Akt and p-mtor proteins in A549 cells were significantly lower in the low-,medium-and high-dose EMB groups than in the control group(all P＜0.05),and the high dose group was lower than the middle dose group,and the middle dose group was lower than the low dose group(all P＜0.05).The apoptosis rate of A549 cells in the low-,medium-and high-dose EmB groups was higher than that in the control group,and showed a dose-dependent manner(all P＜0.05).Conclusion:Phyllanthus emblica extract can inhibit the activity of Akt/mTOR pathway,interfere with the invasion and migration of lung cancer A549 cells,inhibit the rate of subcutaneous tumor formation in nude mice,and induce cell apoptosis.