原发性肺腺癌组织中巨噬细胞CD68+、CD163+的表达与临床病理学特征及预后相关性研究OA

Objective To investigate the expression of macrophage clusters of differentiation(CD)68+ and CD163+ in primary lung adenocarcinoma and their correlation with clinical pathological characteristics and prognosis.Methods This study retro-spectively collected pathological tissue and adjacent tissue samples from 106 patients with primary lung adenocarcinoma who un-derwent surgical and adjuvant chemotherapy in the Department of Cardiothoracic Surgery of the Qingdao Eighth People's Hos-pital from January 2018 to June 2019.Immunohistochemistry was applied to detect and compare the expression levels of CD68+and CD163+ in lung adenocarcinoma tissue and adjacent tissue.Calculated the density of CD68+ and CD163+ macrophages in lung adenocarcinoma tissue andadjacent tissue,cancer nests and adjacent stroma under high magnification,and distinguished high/low density of CD68+/CD163+ in cancer nests/adjacent stroma based on median density.Primary lung adenocarcinoma pa-tients were followed up to measure survival time,and Kaplan-Meier was applied to analyze the impacts of densities of CD68+ and CD163+ macrophages in cancer nests and adjacent stroma on the survival of patients with primary lung adenocarcinoma.Results tissues(χ2=15.881,13.904,all P<0.05).The densities of CD68+ and CD163+ macrophages in lung adenocarcinoma tissues were higher than those in adjacent tissues(χ2=44.143,40.070,all P<0.05).The proportions of stage III-IV lung adenocarcinoma,T3 tumor size,and N2～N3 lymph nodes in the cancer nests and adjacent stroma CD68+ high-density groups were higher than those in the CD68+ low-density groups(χ2=6.788～10.604,all P<0.05).The proportions of stage III～IV lung adenocarcinoma,T3 tumor size,and N2～N3 lymph nodes in the cancer nests and adjacent stroma CD163+ high-density groups were higher than those in the CD163+ low-density groups(χ2=7.556～17.743,all P<0.05).The survival rate of high density of cancer nests CD68+ was 59.32%(35/59),which was lower than 82.98%(39/47)of low density of cancer nests CD68+(χ2=7.332,P<0.05);the survival rate of high density of adjacent stroma CD68+ was 56.36%(31/55),which was lower than 84.31%(43/51)of low density of adjacent stroma CD68+(χ2=9.518,P<0.05).The survival rate of high density of cancer nests CD163+ was 58.93%(33/56),which was lower than 82.00%(41/50)of low density of cancer nests CD163+(χ2=7.137,P<0.05);the survival rate of high density of adjacent stroma CD163+ was 55.56%(30/54),which was lower than 84.62%(44/52)of low density of adjacent stroma CD163+(χ2=12.487,P<0.05).Conclusions The densities of CD68+ and CD163+ macrophages are higher in primary lung adenocarcinoma tissues,and the high densities of CD68+ and CD163+ in cancer nests and adjacent stroma are associated with the progression and infiltration of lung adenocarcinoma,as well as a lower survival rate..