结直肠癌组织中YTHDF1 mRNA和MGP mRNA表达与免疫相关基因的相关性及预后价值分析OA

Objective To study the expression of YTH N6-methyladenosine RNA binding protein 1(YTHDF1)mRNA and Matrix Gla protein(MGP)mRNA in colorectal cancer(CRC)tissues and explore their correlation with immune-related genes and prog-nostic value.Methods A total of 98 patients with CRC diagnosed and treated in Beijing Traditional Chinese Medicine Hospital Inner Mongolia Hospital from February 2019 to February 2021 were selected.Real-time fluorescence quantitative PCR and im-munohistochemistry were used to detect the expression of YTHDF1 and MGP mRNA and protein in tissues.The correlation be-tween YTHDF1 mRNA,MGP mRNA and Programmed death factor 1(PD-1)mRNA,Programmed death factor 1 ligand(PD-L1)mRNA,and interleukin-6(IL-6)mRNA were analyzed by pearson correlation analysis.Kaplan-Meier curve and COX regression were used to analyze the effect of YTHDF1 mRNA and MGP mRNA on the prognosis of CRC.Results The positive rates of YT-HDF1 protein(91.84%vs 7.14%)and MGP protein(89.80%vs 11.22%)in CRC cancer tissues were significantly higher than those in adjacent tissues,the differencse were statistically significant(χ2=140.606,121.013,all P<0.05).Compared with adjacent tissues,the expression of YTHDF1 mRNA(3.11±0.41 vs 1.03±0.22),MGP mRNA(2.89±0.38 vs 0.76±0.20),PD-1 mRNA,PD-L1 mRNA and IL-6 mRNA in CRC cancer tissues were significantly higher(t=26.277～49.103,all P<0.05).There was a posi-tive correlation between YTHDF1 mRNA,MGP mRNA and immune-related genes PD-1 mRNA,PD-L1 mRNA and IL-6 mRNA expression in CRC cancer tissues(r=0.667～0.775,all P<0.05).The expression of YTHDF1 mRNA and MGP mRNA in CRC tis-sues of patients with TNM stage III and lymph node metastasis was significantly higher than that of patients with TNM stage I～II and no lymph node metastasis,the differencse were statistically significant(t=18.825～30.469,all P<0.05).The 3-year survival rate of YTHDF1 mRNA high expression group and low expression group was 45.83%(22/48),72.00%(36/50),and the difference was statistically significant(Log-rank χ2=8.946,P=0.003).The 3-year survival rate of MGP mRNA high expression group and low expression group was 42.55%(20/47),74.51%(38/51),and the difference was statistically significant(Log-rank χ2=12.500,P<0.001).Multivariate COX regression anslysis,YTHDF1 mRNA(OR=1.384,95%CI=1.088～1.761),MGP mRNA(OR=1.324,95%CI=1.080～1.624),TNM stage III and lymph node metastasis were risk factors affecting the prognosis of CRC.Conclusions The elevated mRNA and protein expression of YTHDF1 and MGP in CRC are associated with immune related genes and adverse clinical pathological features.Both of them are tumor markers for evaluating CRC prognosis.