基于代谢组学分析选育系F5代与非选育系马苏大麻哈鱼肌肉代谢物的差异OA

The effect of breeding on muscle metabolites of landlocked Oncorhynchus masou was explored by untar-geted metabolomics technology,which provided a theoretical basis for breeding efforts.Muscle tissues were collected from 10 non selected landlocked O.masou and 10 fifth generation(F5)selected individuals,with untargeted metabolomics studies using liquid-mass coupling(LC-MS)technology.Metabolite differences between F5 selected and non-selected groups were compared,with differential metabolites identified using thresholds of P<0.05,VIP value>1.0,and FC≥1.5(up-regulated)or FC≤0.667(down-regulated).Pathway annotation of the metabolites through KEGG database,phenylpropane biosynthesis and metabolic,fat metabolism,and eicosanoids metabolism pathway were analyzed.Significant differences in muscle metabolites were observed between F5 selected and non-selected groups:compared with the non-selected group,213 differential metabolites were significantly up-regulated and 348 were signifi-cantly down-regulated,mainly annotated to secondary metabolites biosynthesis,bile secretion,and metabolism path-way.KEGG bubble chart analysis showed that phenylpropane biosynthesis,phenylalanine metabolism,and methane metabolism pathway had important effects on muscle growth during selection.Breeding exerted a significant effect on muscle metabolites in landlocked O.masou.Metabolomics analysis showed that post-selection,improvements in muscle nutritional quality relative to the non-selected group may be linked to metabolites changes such as DHA,EPA,AMPK,and PPARα in the phenylpropane biosynthesis and metabolism,fat metabolism,and arachidonic acid metabolism pathway.