基于网络药理学探讨百合固金汤治疗萎缩性鼻炎的作用机制OA

Aim To explore the mechanism of Baihe Gujin Decoction in treating atrophic rhinitis(AR)based on network pharmacology.Methods The active ingredients and potential targets of Baihe Gujin Decoction were screened from TCMSP,Gen-eCards and other drug and AR disease target database,and differential proteomics results.An interaction network of drug-active ingre-dient-potential target was constructed.Potential intersecting targets were enriched and analyzed using Kyoto encyclopedia of genes and genomes(KEGG)and gene ontology(GO).The binding ability between the active ingredient and the core target were verified through molecular docking and molecular dynamics simulation.Results The active ingredients of Baihe Gujin Decoction were screened,including 113 types such as β-sitosterol,quercetin,and kaempferol,with 837 potential drug targets,743 AR targets,and 123 potential intersecting targets.The GO functional enrichment results showed that the targets of Baihe Gujin Decoction were signifi-cantly enriched in Go items such as lipopolysaccharide response,oxidative stress response,cytokine activity;The KEGG pathway anal-ysis identified 163 related pathways,of which the top prominent pathways were lipid and atherosclerosis,fluid shear stress and athero-sclerosis.Neutral sphingomyelinase(F13A1)was a common intersection target of drug targets,disease targets,and differential pro-teomics results.F13A1 has the strongest binding ability with quercetin in Baihe Gujin Decoction.Conclusion The mechanism of action of Baihe Gujin Decoction in treating AR may be related to its rich active ingredients such as β-sitosterol,quercetin,and kaempferol,which alleviate inflammation and improve vascular microcirculation through multi-target and multi pathway mechanisms,thereby inhibiting mucosal atrophy and bone resorption.