96份小麦品种(系)白粉病抗性评价及分子标记检测OA
Resistance Valuation and Molecular Marker Identification of Powdery Mildew Resistance-Related Genes in 96 Wheat Varieties(Lines)
为给小麦抗白粉病育种提供优秀种质资源,在云南、浙江西地通过自然发病和接种鉴定相结合,对96份不同来源小麦品种(系)的成株期白粉病抗性进行了鉴定,并使用已公布的13个抗白粉病基因的分子标记检测供试材料.结果表明,供试材料中,有67份对白粉病表现出抗性,其中高抗材料28份,占29.17％;在62份材料中检测出抗性基因,其中Pm4b的检出频率最高,其次是Pm21;从基因组成和分布上看,携带单基因的材料最多(占比58.1％),仅有1份材料聚合了 5种不同的抗病基因;抗病基因中Pm2、Pm4a、Pm4b、Pm8、Pm18、Pm34、Pm35和Pm52均在部分感病材料中被检测到,说明其抗性可能已被病原菌克服,而Pm21、Pm24、Pm26、Pm57和PmTm4依然持有稳定的抗性.
To provide excellent germplasm resources for wheat breeding against powdery mildew(a ma-jor disease affecting wheat production),this study evaluated the adult plant resistance(APR)of 96 wheat varieties(lines)from diverse sources to powdery mildew under natural infection and artificial in-oculation in Yunnan and Zhejiang.Molecular markers for 13 known Pm genes were used to screen the materials.Results showed that 67 varieties(lines)exhibited resistance,including 28 highly resistant(HR)materials(29.17％).Resistance genes were detected in 62 materials,with Pm4b having the highest detection frequency,followed by Pm21.In terms of gene composition,materials carrying a single resistance gene predominated(58.1％),while only one material combined five different Pm genes.Notably,resistance genes Pm2,Pm4a,Pm4b,Pm8,Pm18,Pm34,Pm35,and Pm52 were detected in some susceptible materials,indicating their resistance may have been overcome by the pathogen.In contrast,Pm21,Pm24,Pm26,Pm57,and PmTm4 retained stable resistance.
王卫东;朱春弟;黄金玲;安曈昕;范敏;华为;朱靖环
浙江省农业科学院作物与核技术利用研究所,浙江 杭州 325005平湖市钟埭街道事业综合服务中心,浙江平湖 314200浙江省农业科学院作物与核技术利用研究所,浙江 杭州 325005||云南农业大学农业与生物技术学院,云南 昆明 650031云南农业大学农业与生物技术学院,云南 昆明 650031浙江省农业科学院作物与核技术利用研究所,浙江 杭州 325005浙江省农业科学院作物与核技术利用研究所,浙江 杭州 325005浙江省农业科学院作物与核技术利用研究所,浙江 杭州 325005
农业科技
小麦白粉病抗性鉴定抗病基因
WheatPowdery mildewResistance identificationResistance genes
《麦类作物学报》 2026 (1)
1-13,13
浙江省农业新品种重大科技专项(2021C02064-3-3)国家自然科学基金项目(32272078)
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