复方加味小柴胡汤对代谢相关脂肪性肝病的疗效及肠道菌群调节作用OA

Objective To explore the effect of Modified Xiaochaihu Decoction(复方加味小柴胡汤)on the intestinal flora of mice with metabolically associated fatty liver disease(MAFLD).Methods Male C57BL/J mice were randomly divided into normal group(NC),model group(HFD),Modified Xiaochaihu Decoction low-dose group(HFD+ZFL),high-dose group(HFD+ZFH)and Polyene phosphatidylcholine group(HFD+PPC),with 10 mice in each group.Except the normal group,the other groups were given high-fat diet induced MAFLD model and different doses of drug intervention for a total of 20 weeks.Body mass,liver wet weight and liver index were detected.Detection of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),interleukin-6(IL-6),interleukin-17(IL-17)by biochemical kit,tumor necrosis factor-α(TNF-α),serum amyloid A(SAA),C-reactive protein(CRP)content;The liver morphology was observed by hematoxylin-eosin(HE)staining.16S ribosomal DNA(16Sr DNA)gene sequencing was used to analyze the changes of intestinal flora in mice.Results The results showed that compared with NC,the body weight and liver wet weight of mice in HFD increased,while the liver index decreased.Serum ALT,AST,TC,TG,LDL-C,HDL-C,IL-6,IL-17,TNF-α and CRP levels were increased.Liver lipid accumulation increased,indicating successful model induction.Compared with HFD,the body weight,liver wet weight and liver index of the mice in HFD+ZFL,HFD+ZFH and HFD+PPC were decreased.Serum levels of ALT,AST,TC,TG,LDL-C,HDL-C,IL-6,IL-17,TNF-α and CRP of mice in HFD+ZFL,HFD+ZFH and HFD+PPC were decreased.The degree of hepatic steatosis of mice in HFD+ZFL,HFD+ZFH and HFD+PPC were reduced.Intestinal flora analysis showed that compared with HFD,Modified Xiaochaihu Decoction could improve the richness and diversity of intestinal flora in mice.At the phylum level,Bacteroidetes and Actinomycetes were increased in MAFLD mice.At the genus level,it plays a major role in increasing the abundance of Bifidobacterium,Lactobacillus and Bacteroides_H.Conclusion Modified Xiaochaihu Decoction can treat MAFLD by reducing lipid accumulation,inhibiting inflammation and regulating intestinal flora homeostasis.