化痰通络法对咳嗽变异性哮喘豚鼠气道重塑相关细胞因子的影响OA

Objective To study the effects of method of eliminating phlegm and dredging collaterals on a cytokines related to airway remodeling in guinea pigs with cough variant asthma(CVA),and to explore the mechanism of treating CVA with method of eliminating phlegm and dredging collaterals,so as to provide evidence for clinical treatment.Methods Forty guinea pigs were randomly divided into normal group,model group,Montolukast sodium group(MS group)and Huatan Tongluo group.The CVA guinea pig model was replicated by ooprotein,aluminum hydroxide sensitization and inhalation ooprotein stimulation.The pathological manifestations of lung tissue and interleukin-17(IL-17),transform growth factor β1(TGF-β1),vascular endothelial growth factor(VEGF)and platelet-derived growth factor(PDGF).Results Macroscopic observation:the color of lung tissue in the model group was dark red,with peechiae and ecchymosis in some cases,dull edges,hyperemia and edema,and increased volume.The color of lung tissue in the drug intervention group was lighter than that in the model group,and the degree of hyperemia and edema was lighter.HE staining:In the model group,bronchial basement membrane was significantly thickened,hyperemia and edema were obvious,lumen became smaller,mucus thrombus was visible,inflammatory cell infiltration was obvious,bronchial epithelial cell arrangement was disordered,and alveolar wall was destroyed.Compared with the model group,the bronchial basement membrane of guinea pigs in the drug intervention group was thinner,the degree of congestion and edema was lighter,the mucus thrombus was reduced,the lumen was relatively complete and unobstructed,the inflammatory cell infiltration was lighter,and the alveolar lumen was clearer.Airway morphological parameters of each group:There was no significant difference in bronchial basal membrane circumference(Pbm)among all groups(P＞0.05),total tube wall area(Wat)in model group was increased compared with normal group(P＜0.05),Wat in Huatan Tongluo group was decreased compared with model group(P＜0.05),and tube wall smooth muscle area(Wam)in model group was increased compared with normal group(P＜0.05),Wam in Huatan Tongluo group was decreased compared with model group(P＜0.05).The Wat/Pbm and Wam/Pbm in Huatan Tongluo group were increased compared with the normal group(P＜0.05),and the WAT/PBM and WAM/PBM in Huatan Tongluo group were decreased compared with the model group(P＜0.05).Cytokines:The levels of IL-17,TGF-β1,VEGF and PDGF in serum and bronchoalveolar lavage fluid(BALF)in model group were higher than those in normal group(P＜0.05),while those in Huatan Tongluo group were lower than those in model group(P＜0.05).Conclusion Method of eliminating phlegm and dredging collaterals may reduce airway remodeling by regulating the levels of IL-17,TGF-β1,VEGF and PDGF.