早期胃癌组织中PDE1B、BMAL1蛋白表达情况与经内镜黏膜下剥离术后复发的关系OA

Objective To investigate the relationship between phosphodiesterase 1B(PDE1B),brain and muscle aryl hydrocarbon receptor nuclear transporter like 1(BMAL1)protein expression and recurrence of early gastric cancer after endoscopic submucosal dissection(ESD).Methods A total of 155 patients with gas-tric cancer who underwent ESD in this hospital from January 2018 to December 2021 were selected as the re-search objects.Followed up for 3 yesrs all gastric cancer patients were divided into recurrence group and non-recurrence group according to whether they had recurrence after ESD.The expression of PDE1B and BMAL1 protein in early gastric cancer tissues and adjacent tissues was detected by immunohistochemistry.The recur-rence-free survival rates of patients with positive and negative PDE1B and BMAL1 protein expression after ESD were compared by K-M survival curve,and Log-rank χ2 test was performed.Multivariate Cox regression analysis was used to analyze the influencing factors of recurrence after ESD in gastric cancer patients.Results The positive expression rate of PDE1B protein in gastric cancer tissues was higher than that in adja-cent tissues,and the positive expression rate of BMAL1 protein was lower than that in adjacent tissues,and the differences were statistically significant(P<0.05).After 3 years of follow-up,45(29.03%)of 155 gas-tric cancer patients relapsed after ESD.The positive expression rate of cancer tissue PDE1B protein in the re-currence group was higher than that in the non-recurrence group,and the positive expression rate of BMAL1 protein in the recurrence group was lower than that in the non-recurrence group,and the differences were sta-tistically significant(P<0.05).The results of K-M survival curve analysis showed that the recurrence-free survival curve of PDE1B protein positive patients after ESD was lower than that of PDE1B protein negative patients(Log-rank χ2=23.290,P<0.001).The recurrence-free survival curve of patients with positive BMAL1 protein expression after ESD was higher than that of patients with negative BMAL1 protein expres-sion(Log-rank χ2=7.676,P=0.006).Multivariate Cox regression analysis showed that poor differentiation and positive expression of cancer tissue PDE1B protein were independent risk factors for recurrence of gastric cancer patients after ESD(P<0.05),and positive expression of cancer tissue BMAL1 protein was an inde-pendent protective factor for recurrence of early gastric cancer patients after ESD(P<0.05).Conclusion The posi-tive expression rate of PDE1B protein in gastric cancer tissues is increased,and the positive expression rate of BMAL1 protein is decreased,which is closely related to the recurrence of gastric cancer after ESD.